FISH: Small Target Protocol

Small Target Fluorescent in Situ Hybridization in Plants

A companion video emphasizing fluorescence in situ hybridization (FISH) methods used to detect single genes and other small targets ranging in size from 2-7 Kb.

The following two companion resources contain recipes, probe plasmid data, and other information needed for doing the procedures illustrated in this video.

An Introduction to FISH Video:
Maize Karyotyping and FISH Manual 2015:…
Chromosome Painting Article PNAS:…