FISH: Large Target Protocol

An Introduction to Fluorescence in Situ Hybridization
and Karyotype Analysis in Plants

A video protocol and description of Fluorescence In Situ Hybridization (FISH) in plants.

The following two companion resources contain recipes, probe plasmid data, and other information needed for doing the procedures illustrated in this video. Of further interest are suggestions for developing a karyotyping cocktail for other plant species and making probes from BAC DNA.

Resource Links:
Maize Karyotyping and FISH Manual 2015
Maize Somatic Chromosome Painting