Figure 1: MtDNA segments hybridized to B73 chromosomes. (A). Linear map of the NB maize mitochondrial genome with cosmid locations. A linearized version of the 569,630 bp NB maize mitochondrial genome is shown (CLIFTON et al. Physiol. 2004) (condensed from (ALLEN et al. Genetics 2007)) with the cosmid positions outlined. Mitochondrial genes, rRNAs, and tRNAs (half-length lines) are represented by vertical lines. Repeats are indicated by colored arrows. The cpDNA insertions are indicated by green rectangles. A scale in kilobases is shown along the bottom. (B) Individual mtDNA-containing cosmids hybridized to B73 chromosomes. Individual cosmid probes containing mtDNA were labeled with Texas Red and hybridized to B73 chromosomes. The white arrowheads mark mtDNA insertion sites and the green arrowheads mark potential cpDNA insertion sites. The eight-mix of karyotyping probes (KATO et al. 2004) was used to identify each chromosome. Only the layer with the Texas Red-labeled mitochondrial probes is shown. The marked mtDNA insertion sites were identified on > 88% of individual chromosomes examined.